goat anti- keap1  (Servicebio Inc)

Journal: International Journal of Molecular Sciences

Article Title: Melatonin Promotes the Development of Secondary Hair Follicles in Adult Cashmere Goats by Activating the Keap1-Nrf2 Signaling Pathway and Inhibiting the Inflammatory Transcription Factors NFκB and AP-1

doi: 10.3390/ijms24043403

Figure Lengend Snippet: Schematic representation of the mode of action of melatonin on the promotion of secondary hair follicle growth and development. MT-melatonin, OS-oxidative stress, CAT -catalase, T-AOC -total antioxidant capacity, SOD -superoxide dismutase, GSHPX/GPx -glutathione peroxidase, MDA -malondialdehyde, SHF-secondary hair follicle, Keap1 -Kelch-like ECH-associated protein 1, Nrf2 -nuclear factor erythroid 2-related factor 2, NFκB -nuclear factor kappa B, SASP-senescence-associated phenotype, IL -interleukin, TIMP -tissue inhibitor of metallo proteinases, CXL/CCL -chemokine.

Article Snippet: The PVDF membrane was blocked with 5% skimmed cow’s milk, followed by rabbit anti- Nrf2 (1:2000), goat anti- Keap1 (1:2000), rabbit anti- P65 (1:2000), rabbit anti- C-fos (1:1000), rabbit anti- C-Jun (1:1000), rabbit anti- β-actin (1:6000) (Servicebio, Wuhan, China) overnight at 4 °C, then incubated with secondary antibodies.

Techniques:

Journal: Molecular Cancer

Article Title: Nrf2 is overexpressed in pancreatic cancer: implications for cell proliferation and therapy

doi: 10.1186/1476-4598-10-37

Figure Lengend Snippet: Basal expression levels of Keap1, Nrf2 and Nrf2-regulated genes GCLC and AKR1c1 and GSH amongst a panel of pancreatic cancer cell lines . A, Immunoblot detection of basal Keap1 protein in MiaPaca-2, Panc-1, FAMPAC, Paca-2 and Suit-2 cells. B, Immunoblot detection of basal Nrf2 in cells untreated or treated with the proteosome inhibitor MG132 (10 μM) for 2 h in order to visualize Nrf2 protein. The band labelled 'non-specific' was not depleted following transfection with 10 nM Nrf2-targeting siRNA Nrf2 (data not shown). Beta actin was used as a reference control for blots A and B. C, Immunoblot detection of basal GCLC and AKR1c1. D, Total basal glutathione levels. * = P < 0.05,** = P < 0.01,*** = P < 0.001.

Article Snippet: Goat anti-Keap1 and rabbit anti-Nrf2 primary antibodies were purchased from Santa Cruz (Heidelberg, Germany). siRNA targeted against Nrf2 was purchased from Dharmacon (Lafayette, USA).

Techniques: Expressing, Western Blot, Transfection

Journal: Molecular Cancer

Article Title: Nrf2 is overexpressed in pancreatic cancer: implications for cell proliferation and therapy

doi: 10.1186/1476-4598-10-37

Figure Lengend Snippet: Immunohistochemical analysis of Nrf2 and Keap1 in pancreatic tissues . A and B, Pancreatic cancer tissue showing strong and weak Nrf2 levels, respectively. E and F, Pancreatic cancer tissue showing Keap1 expression. C and G, Pancreatic cancer tissue showing absence of detectable Nrf2 and Keap1, respectively. D and H, Benign pancreatic tissue showing Nrf2 expression in ductal cells and acinar cells (D) and absence of Keap1 in ductal cells, with positive islet cells (H). Scale bars = 50 μm.

Article Snippet: Goat anti-Keap1 and rabbit anti-Nrf2 primary antibodies were purchased from Santa Cruz (Heidelberg, Germany). siRNA targeted against Nrf2 was purchased from Dharmacon (Lafayette, USA).

Techniques: Immunohistochemical staining, Expressing